The following points highlight the fifteen main factors affecting photosynthesis. Carbon Dioxide Concentrations 3. Accumulation of Carbohydrates The reason being that all the reactions of the Calvin cycle are temperature dependent and the rate of diffusion of CO2 to the chloroplasts is accelerated by high temperature.
Measuring the rate of photosynthesis Measuring the rate of photosynthesis Without photosynthesis life as we know it would not exist. Plant biomass is the food and fuel for all animals.
Plants are the primary producers.
These amazing organisms are capable of capturing the energy of sunlight and fixing it in the form of potential chemical energy in organic compounds. The organic compounds are constructed from two principle raw materials; carbon dioxide and water which is a source of hydrogen.
These compounds are stable and can be stored until required for life processes. Hence animals, fungi and non-photosynthetic bacteria depend on these for the maintenance of life.
But how can we measure the rates at which photosynthesis takes place? The quantities are mind boggling. There is a total of x tonnes of carbon dioxide in the atmosphere and photosynthesis fixes x tonnes per year.
What are the different methods of measuring the rate of photosynthesis? There are a few key methods to calculate the rate of photosynthesis.
Read the full protocol on using immobilsed algae to measure photosynthesis. Using a CO2 monitor - More simply, you could put a plant in a plastic bag and monitor the CO2 concentration in the bag using a CO2 monitor.
Naturally, the soil and roots must NOT be in the bag as they respire. Alternatively, you could place some Bicarbonate Indicator Solution in the bag with the plant and watch the colour change. This would best be done with a reference colour chart to try to make the end-point less subjective.
This could give a comparison between several plants. The leaf area of the plants should be measured so you can compensate for plant size.
Atmospheric air is only ppm CO2, so there is not much CO2 to monitor and the plant will soon run out of CO2 to fix. Measuring photosynthesis via the production of oxygen Oxygen can be measured by counting bubbles evolved from pondweed, or by using the Audus apparatus to measure the amount of gas evolved over a period of time.
To do this, place Cabomba pondweed in an upside down syringe in a water bath connected to a capillary tube you can also use Elodea, but we find Cabomba more reliable. Put the weed in a solution of NaHCO3 solution. You can then investigate the amount of gas produced at different distances from a lamp.
Read a full protocol on how to investigate photosynthesis using pondweed. Measuring photosynthesis via the production of carbohydrates There is a crude method where a disc is cut out of one side of a leaf using a cork borer against a rubber bung and weighed after drying.
Some days or even weeks latera disk is cut out of the other half of the leaf, dried and weighed. Increase in mass of the disc is an indication of the extra mass that has been stored in the leaf. This is very simple to do and enables you to investigate plants growing in the wild.
However, you can probably think of several inaccuracies in this method. If you harvest several plants and record how much mass they have accumulated you will have an accurate measure of the surplus photosynthesis over and above the respiration that has taken place.
As with most methods, you need several plants so you have replicate measurements and you can find an average and a standard deviation if necessary. Investigating the light-dependent reaction in photosynthesis The rate of decolourisation of DCPIP in the Hill Reaction is a measure of the rate of the light-requiring stages of photosynthesis.Student Exploration: Photosynthesis Lab Vocabulary: carbon dioxide, chlorophyll, glucose, limiting factor, you can monitor the rate of photosynthesis by measuring oxygen production.
1. Observe the left pane closely. change causes oxygen production to increase, continue to experiment. If necessary, revise your numbers in the table above.
Based on our data and research, we can determine that temperature affects the rate of photosynthesis in plants. Plants have an optimum temperature for photosynthesis, which varies.
The experiment could have been carried out with higher NaHCO3 to see if increasing the concentration would increase the rate of photosynthesis, or if a concentration of M NaHCO3 produces the maximum rate of photosynthetic reaction. Under laboratory conditions when light and temperature are not limiting factors, increase in CO 2 concentration in the atmosphere from % to % raises rate of photosynthesis. With the further increase in the concentration of CO 2 progressively the rate of carbon assimilation increases slightly and then it becomes independent of CO . This activity is a lab where students design an experiment to test the rate of photosynthesis. Students will analyze data,write a report using the scientific method, and apply results to current environmental issues. learn that the rate of photosynthesis is influenced by environmental factors that can be quantified Students should be.
This is the temperature at which the rate is the fastest. If the temperature is decresed, then so is the rate of photosynthesis.
This activity will allow students to measure the rate at which the photosynthesis process occurs. Students will work in small groups to design an experiment with one independent variable and test this variable on spinach leaf disks.
A brief outline of various ways to measure the rate of photosynthesis - including measuring photosynthesis via the uptake of carbon dioxide (e.g. using immobilised algae), via the production of oxygen in pondweed, via the increase in dry mass, and via the production of carbohydrates.
But how can we measure the rates at which photosynthesis takes place? The quantities are mind boggling. A hectare (e.g. a field m by m) of wheat can convert as much as 10, kg of carbon from carbon dioxide into the carbon of sugar in a year, giving a total yield of 25, kg of sugar per year. The reactions of photosynthesis can be divided into two major types: light-dependent reactions and light-independent reactions.
The light-dependent reactions convert energy from the sun into a form that the chloroplast can then use to make sugar from carbon dioxide, in the process producing oxygen as a .